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Literature summary extracted from
- Sulfolobus solfataricus protein disulphide oxidoreductase: insight into the roles of its redox sites (2009), Protein Eng. Des. Sel., 22, 19-26.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.1.B4 | wild-type enzyme and mutant enzymes C155S/C158S, C41S/C44S and C173S/C178S are expressed in Escherichi coli | Saccharolobus solfataricus |
| 1.8.99.B1 | wild-type enzyme and mutant enzymes C155S/C158S, C41S/C44S and C173S/C178S are expressed in Escherichi coli | Saccharolobus solfataricus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.8.99.B1 | C155S/C158S | with insulin as substrate the enzyme is completely inactive | Saccharolobus solfataricus |
| 1.8.99.B1 | C173S/C178S | with insulin as substrate the enzyme shows lower activity than wild-type enzyme | Saccharolobus solfataricus |
| 1.8.99.B1 | C41S/C44S | with insulin as substrate the enzyme shows lower activity than wild-type enzyme | Saccharolobus solfataricus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.1.B4 | 0.00012 | - |
[C173S/C178S mutant of protein-disulfide-oxidoreductase]-disulfide | pH 7.0, 60°C | Saccharolobus solfataricus | |
| 1.8.1.B4 | 0.00017 | - |
[C41S/C44S mutant of protein-disulfide-oxidoreductase]-disulfide | pH 7.0, 60°C | Saccharolobus solfataricus | |
| 1.8.1.B4 | 0.0002 | - |
[protein disulfide oxidoreductase]-disulfide | pH 7.0, 60°C | Saccharolobus solfataricus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.B4 | Saccharolobus solfataricus | Q97W27 | - |
- |
| 1.8.1.B4 | Saccharolobus solfataricus P2 | Q97W27 | - |
- |
| 1.8.99.B1 | Saccharolobus solfataricus | Q980T5 | - |
- |
| 1.8.99.B1 | Saccharolobus solfataricus P2 | Q980T5 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.B4 | [C173S/C178S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+ | the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase | Saccharolobus solfataricus | [C173S/C178S mutant of protein-disulfide-oxidoreductase]-dithiol + NADP+ | - |
? |  |
| 1.8.1.B4 | [C173S/C178S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+ | the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase | Saccharolobus solfataricus P2 | [C173S/C178S mutant of protein-disulfide-oxidoreductase]-dithiol + NADP+ | - |
? | |
| 1.8.1.B4 | [C173S/C178S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+ | the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase | Saccharolobus solfataricus | [C173S/C178S mutant ofprotein-disulfide-oxidoreductase]-dithiol + NADP+ | - |
? | |
| 1.8.1.B4 | [C173S/C178S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+ | the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase | Saccharolobus solfataricus P2 | [C173S/C178S mutant ofprotein-disulfide-oxidoreductase]-dithiol + NADP+ | - |
? | |
| 1.8.1.B4 | [C41S/C44S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+ | the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase | Saccharolobus solfataricus | [C41S/C44S mutant of protein disulfide oxidoreductase]-dithiol + NADP+ | - |
? | |
| 1.8.1.B4 | [C41S/C44S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+ | the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase | Saccharolobus solfataricus P2 | [C41S/C44S mutant of protein disulfide oxidoreductase]-dithiol + NADP+ | - |
? | |
| 1.8.1.B4 | [C41S/C44S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+ | the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase | Saccharolobus solfataricus | [C41S/C44S mutant ofprotein disulfide oxidoreductase]-dithiol + NADP+ | - |
? | |
| 1.8.1.B4 | [C41S/C44S mutant of protein-disulfide-oxidoreductase]-disulfide + NADPH + H+ | the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase | Saccharolobus solfataricus P2 | [C41S/C44S mutant ofprotein disulfide oxidoreductase]-dithiol + NADP+ | - |
? | |
| 1.8.1.B4 | [protein disulfide oxidoreductase]-disulfide + NADPH + H+ | the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase | Saccharolobus solfataricus | [protein disulfide oxidoreductase]-dithiol + NADP+ | - |
? | |
| 1.8.1.B4 | [protein disulfide oxidoreductase]-disulfide + NADPH + H+ | the enzyme is able to reduce mutant protein-disulfide-oxidoreductase C41S/C44S and mutant protein-disulfide-oxidoreductase C173S/C178S with an affinity comparable with the wild-type protein-disulfide-oxidoreductase | Saccharolobus solfataricus P2 | [protein disulfide oxidoreductase]-dithiol + NADP+ | - |
? | |
| 1.8.99.B1 | [insulin]-disulfide + reduced dithiothreitol | - |
Saccharolobus solfataricus | [insulin]-dithiol + oxidized dithiothreitol | - |
? | |
| 1.8.99.B1 | [insulin]-disulfide + reduced dithiothreitol | - |
Saccharolobus solfataricus P2 | [insulin]-dithiol + oxidized dithiothreitol | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.1.B4 | SSO2416 | locus name | Saccharolobus solfataricus |
| 1.8.1.B4 | SsTR | - |
Saccharolobus solfataricus |
| 1.8.99.B1 | protein disulfide oxidoreductase | - |
Saccharolobus solfataricus |
| 1.8.99.B1 | SSO0192 | locus name | Saccharolobus solfataricus |
| 1.8.99.B1 | SsPDO | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.B4 | 60 | - |
assay at | Saccharolobus solfataricus |
| 1.8.99.B1 | 30 | - |
assay at | Saccharolobus solfataricus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.B4 | 7 | - |
assay at | Saccharolobus solfataricus |
| 1.8.99.B1 | 7 | - |
assay at | Saccharolobus solfataricus |
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Release 2023.1 (January 2023)
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